Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining
نویسندگان
چکیده
منابع مشابه
Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining
Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incub...
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Immunocytochemistry on sectioned tissue is a powerful method for determining the cellular and subcellular distributions of an epitope. However, where the expression is patterned, understanding the distribution often requires extensive, time-consuming threedimensional reconstruction with all its attendant artifacts. The techniques described in this unit circumvent these problems. The distributio...
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Background and Objective: Hodgkin's lymphoma is a potentially curable hematologic malignancy with difficulty in its diagnosis especially in atypical cases even in expert hands. Today, immunohistochemistry plays a significant role in the diagnosis of it especially applying the anti-CD15 and anti-CD30 antibodies. The negativity of CD15 can be reduced by antigen retrieval for meth...
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The molecule 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is a fluorescent dye which diffuses within cell membranes. The properties of DiI diffusion and fluorescence are maintained in aldehyde-fixed tissue, thereby allowing selective neuronal tracing post mortem. We describe three modifications of this tracing method. First, while DiL diffuses along neuronal membrane...
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ژورنال
عنوان ژورنال: International Journal of Cell Biology
سال: 2016
ISSN: 1687-8876,1687-8884
DOI: 10.1155/2016/7076910